F4049

Immunofluorescence
COS 7 cells transfected with pFLAG-CMV2-BAP plasmid stained with ANTI-FLAG M2 FITC Conjugate (Cat No. F4049).

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F4049

Sigma

 

Monoclonal ANTI-FLAG® M2-FITC Conjugate antibody produced in mouse

clone M2, purified immunoglobulin, buffered aqueous solution

Synonym:Monoclonal ANTI-FLAG® M2 antibody produced in mouse

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Description

ApplicationFor simple, one-step detection by immunocytochemistry. Especially useful in detection of FLAG fusion proteins expressed in murine host, where secondary anti-mouse antibodies may cause cross-reactivity.
Other NotesTo view FLAG® technology literature, visit www.sigma-aldrich.com/flagliterature.
SpecificityBinds to the FLAG® epitope wherever it is located in the fusion protein: amino-terminal, Met-amino-terminal, carboxy-terminal, or internal. Binding is not Ca2+-dependent.
Physical formSolution in 10 mM phosphate buffered saline, plus 1% BSA and 0.1% sodium azide, pH 7.4
Legal InformationANTI-FLAG is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.
 FLAG is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Properties

antibody formpurified immunoglobulin
cloneM2 (monoclonal)
formbuffered aqueous solution
application(s)direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone
isotypeIgG1
shipped indry ice
storage temp.−20°C

Safety

Hazard CodesXn
Risk Statements22
WGK GermanyNWG